Journal: Nature Communications

Article Title: Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases

doi: 10.1038/s41467-021-26367-9

Figure Lengend Snippet: a ID Salmonella delivery of NIPP1-CD caused more death ( red , white arrows ) in Hepa 1-6 cells compared to controls ( P = 0.0021, n = 6). b In microfluidic tumor masses, delivery of NIPP1-CD caused more cell death ( red ) than bacterial controls. The percentage of dead cells increased with time as Salmonella invaded into cells and delivered protein ( P = 0.042, 0.017, 0.014, 0.017, 0.024, 0.030, and 0.039 at 6.5, 7, 7.5, 8, 8.5, 9, and 9.5 h, respectively, n = 4). c For multiple ( n = 4) tumor masses, as shown in b , NIPP1-CD significantly increased cell death ( P = 0.0177). d NIPP1-CD ID Salmonella was intravenously administered to BALB/c mice with subcutaneous 4T1 tumors. After 31 days, Salmonella ( black arrow , green ) and delivered NIPP1-CD ( white arrows , red ) were dispersed throughout the tissue. Data are shown as means ± SEM. Statistical comparisons in a – c are two-tailed, unpaired Student’s t -tests with asterisks indicating significance (* P < 0.05; ** P < 0.01). Images in a, b , and d are representative of 6, 4, and 3 independent biological samples, respectively. Scale bars in a and b are 100 µm, and in d the scale bar is 10 µm.

Article Snippet: After 31 days, tumors were excised and stained for Salmonella ( Abcam , catalog # ab69253; 1:100 dilution) and NIPP1-CD with antibodies to the c-terminal myc tag ( Chromotek , catalog # 9e1-100; 1:100 dilution), followed by a secondary antibody to the myc specific antibody ( Life Technologies , catalog # A11077; 1:100 dilution).

Techniques: Two Tailed Test

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–F ) Wild-type border cell migration during oogenesis stages 9 and 10. ( A–C ) Egg chambers at the indicated stages labeled with E-Cadherin (E-Cad; green), F-actin (magenta) and DAPI (blue). Arrowheads indicate the border cell cluster. ( D–F ) Magnified views of the same border cell cluster from ( A–C ), showing FasIII (red) in the polar cells, E-Cad and DAPI. The border cell cluster is composed of two polar cells (marked by asterisks) in the center and four to eight outer border cells that are tightly connected with each other as indicated by E-Cad staining. ( G, H ) Egg chambers labeled with Singed (SN; green) to detect border cells (arrowheads), phalloidin to detect F-actin (red), and DAPI to detect nuclei (blue). Control border cells ( G ) reach the oocyte as a single cluster, whereas NiPp1-expressing border cells ( H ) dissociate from the cluster into small groups, with only a few reaching the oocyte. ( I ) Quantification of border cell cluster migration for matched control and NiPp1 overexpression, shown as the percentage that did not complete (red), or completed (green) their migration to the oocyte, as indicated in the egg chamber schematic. ( J ) Quantification of cluster cohesion, shown as the percentage of border cells found as a single unit (one part) or split into multiple parts (2–3 parts or >3 parts) in control versus NiPp1-expressing egg chambers. ( I, J ) Error bars represent SEM in three experiments, each trial assayed n ≥ 69 egg chambers (total n ≥ 221 egg chambers per genotype). ***p<0.001, ****p<0.0001, unpaired two-tailed t test. ( K–L’’ ) Frames from a control ( ; K–K” ) and an NiPp1 overexpression (OE; ; L–L” ) time-lapse video showing movement of the border cell cluster over the course of 3 hr (time in minutes). Border cells (arrowheads) express UAS-mCherry-Jupiter, which labels cytoplasmic microtubules. ( M ) Measurement of border cell migration speed from control (n = 11 videos) and NiPp1 overexpression (n = 11 videos; 22 tracked border cell ‘parts’) videos, shown as a box-and-whiskers plot. The whiskers represent the minimum and maximum; the box extends from the 25th to the 75th percentiles and the line indicates the median. ****p<0.0001, unpaired two-tailed t test. In this and all subsequent figures, anterior is to the left and the scale bars indicate the image magnification. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Migration, Labeling, Staining, Control, Expressing, Over Expression, Two Tailed Test

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A ) Fertility of control versus NiPp1-expressing females. The average progeny per female in each vial (individual plot points) is shown as a box-and-whiskers plot (see legend for details of plot). ( B ) Expression pattern of c306 -GAL4 in the border cell cluster, as visualized by driving the expression of the membrane marker UAS-PLCδ-PH-EGFP (green). Nuclei are labeled by DAPI (blue). The central polar cells (asterisk) express GFP. ( C ) Schematic drawing of the border cell cluster showing the patterns of GAL4 drivers. upd -GAL4 (green) is used to drive expression in polar cells; slbo -GAL4 (blue) is used to drive expression in outer border cells but not polar cells. ( D–G ) Overexpression of NiPp1 in border cells, driven by slbo- GAL4, disrupts border cell cluster migration and cohesion. ( D, E ) Stage 10 slbo- GAL4 egg chambers expressing mCD8-GFP (green), which is detected in border cells (arrowheads), and stained for DAPI to label nuclei (blue) and phalloidin to label F-actin (red, ( D ). Control border cells ( D ) reach the oocyte as a single unit, but NiPp1 overexpressing border cells ( E ) dissociate from the cluster and fail to reach the oocyte. ( F ) Quantification of border cell migration for matched control and NiPp1 overexpression, shown as the percentage that did not complete (red), or completed (green) their migration to the oocyte (see for egg chamber schematic). ( G ) Quantification of cluster cohesion, shown as the percentage of border cells found as a single unit (one part) or split into multiple parts (2–3 parts or >3 parts) in control versus NiPp1-expressing egg chambers. ( F, G ) ****p<0.0001; unpaired two-tailed t test. Error bars represent SEM in three experiments, each trial assayed n ≥ 62 egg chambers (total n ≥ 201 for each genotype). ( H–K ) NiPp1 overexpression in polar cells, driven by upd- GAL4, does not impair border cell cluster migration or cohesion. ( H, I ) Stage 10 upd- GAL4 egg chambers expressing mCD8-ChRFP (red in H) or NiPp1-HA (red in I), which are detected in the polar cells (arrowheads), and co-stained for phalloidin to detect F-actin (magenta), SN to detect border cells (green), and DAPI to label nuclei (blue). ( J, K ) Quantification of border cell migration ( J ) and border cell cluster cohesion ( K ) for matched control and NiPp1 overexpression. Error bars represent SEM in three experiments, each trial assayed n ≥ 80 egg chambers (total n ≥ 313 for each genotype). ****p<0.0001, unpaired two-tailed t test. ( L–N ) Border cells expressing NiPp1 driven by c306- GAL4 ( M, N ), can separate from the polar cells, whereas control border cells ( L ) stay attached to polar cells. Stage 10 egg chambers stained for SN (green) to detect border cells, FasIII (red) to detect polar cells (arrows), and DAPI to label nuclei (blue). Yellow dashed line indicates anterior border of the oocyte. Example of a control ( L ) and two representative NiPp1 overexpressing border cell clusters ( M, N ). All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Control, Expressing, Membrane, Marker, Labeling, Over Expression, Migration, Staining, Two Tailed Test

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–B’ ) Stage 10 wild-type ( A, A’ ) and NiPp1-expressing ( B, B’ ) egg chambers stained with anti-Slbo (green in A, B; white in A’, B’ ), anti-Eya (red in A, B ), and DAPI to detect nuclei (blue in A, B ). Eya primarily marks the anterior follicle cells with lower levels in border cells but is absent from polar cells. Slbo marks border cells and polar cells (arrowheads). Insets, zoomed-in images of Slbo-expressing cells. ( C ) Quantification of cell number per cluster in control and NiPp1-expressing border cell clusters. The total number of egg chambers scored for cell number in each genotype is shown and was assayed in three independent trials. Error bars represent SEM, *p<0.05, unpaired two-tailed t test. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Expressing, Staining, Control, Two Tailed Test

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–F ) Stage 9 and 10 egg chambers showing the endogenous patterns of Pp1c subunits (green) in border cells (arrowheads), follicle cells, and the germline nurse cells and oocyte. DAPI (blue) labels nuclei. Insets, zoomed-in detail of border cells from the same egg chambers. ( A–C ) Pp1α−96A (green) expression, visualized by a GFP-tagged fly-TransgeneOme (fTRG) line. ( D–F ) Flw expression (green), visualized by a YFP-protein trap in the endogenous flw genetic locus. ( G, H ) Overexpression of Pp1c genes rescues the migration ( G ) and cluster cohesion ( H ) defects of NiPp1-expressing border cells. ( G ) Quantification of the migration distance at stage 10 for border cells in NiPp1-expressing egg chambers versus rescue by overexpression of the indicated Pp1c genes, shown as complete (green) and incomplete (red) border cell migration (see for egg chamber schematic). ( H ) Quantification of cluster cohesion at stage 10, shown as the percentage of border cells found as a single unit (one part) or split into multiple parts (two parts, three parts,>3 parts) in NiPp1-expressing egg chambers versus rescue by overexpression of the indicated Pp1c genes. ( G, H ) Error bars represent SEM in three experiments, each trial assayed n ≥ 44 egg chambers (total n ≥ 148 per genotype). *p<0.05, **p<0.01; ***p<0.001; ****p<0.0001, unpaired two-tailed t test. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Expressing, Over Expression, Migration, Two Tailed Test

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–E ) Overexpression of Pp1c subunits on their own does not impair border cell migration to the oocyte. Stage 10 egg chambers of the indicated genotypes stained for Armadillo (Arm; β-Catenin) to detect cell membranes (green in A-D) or SN to detect border cells (green in E), HA to detect Pp1c overexpression (red in A-D) and DAPI to label nuclei (blue). ( F–K ) Overexpression of Pp1c subunits can rescue NiPp1-induced border cell migration defects and cohesion. Stage 10 egg chambers of the indicated genotypes stained for SN to detect border cells (green in G-K) or phalloidin to detect F-actin (green in F, red in G-K), mCD8-ChRFP (red in F), and DAPI to label nuclei (blue). Border cells in all panels are indicated by arrowheads. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Over Expression, Migration, Staining

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–A” ) NiPp1-HA overexpression alone localizes mainly in the nuclei, revealed by HA antibody (red in A, A’ ) and human NiPp1 antibody (PPP1R8; green in A, A’’ ). ( B–C” ) NiPp1-HA overexpression promotes the nuclear localization of two Pp1c subunits, of Flw and Pp1α−96A. Stage 10 egg chambers co-expressing Flw-YFP (green in B, B” ) or Pp1α−96A-GFP (green in C, C” ) with UAS-NiPp1 were stained for anti-HA to detect NiPp1 expression (red in B, B’, C, C’ ) and DAPI to detect nuclei (blue in B, C; white in B’, B”, C’, C” ). Insets, zoomed-in images of border cells.

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Over Expression, Expressing, Staining

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–J ) Knocking down E-Cad , β-Cat or α-Cat by RNAi disrupts border cell cluster migration and cohesion. Images of stage 10 egg chambers stained for phalloidin to label F-actin (red) and DAPI to label nuclei (blue). Border cells (arrowheads) express the membrane marker PLCδ-PH-EGFP (green). ( E–J ) Quantification of border cell migration ( E, G, I ) and cluster cohesion ( F, H, J ) in stage 10 control and E-Cad-RNAi ( E, F ), β-Cat-RNAi ( G, H ) and α-Cat-RNAi ( I, J ) egg chambers. The controls for E-Cad and β-Cat-RNAi are identical, but shown on separate graphs ( E–H ) for clarity; a separate matched control is shown for α-Cat RNAi ( I, J ). Error bars represent SEM in three experiments, each trial assayed n ≥ 27 egg chambers (total n ≥ 93 for each genotype ). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001, unpaired two-tailed t test. ( E, G, I ) Quantification of border cell migration, shown as the percentage of egg chambers with complete (green), partial (blue), or no (red), border cell migration. ( F, H, J ) Quantification of cluster cohesion, shown as the percentage of border cells found as a single unit (one part) or split into multiple parts (2–3 parts or >3 parts) in control versus RNAi egg chambers. ( K–N’’ ) Representative images showing the E-Cad (white in K, L; green in K’', L” ) and β-cat (white in M, N; green in M’', N” ) protein expression pattern in control and NiPp1 overexpressing (OE) border cells. Border cells were co-stained for DAPI to mark nuclei (white in K’, L’, M’, N’; blue in K’', L”, M”, N” ). Images were generated from merged z -sections. The enriched levels of E-Cad ( K, L ) and β-cat ( M, N ) between border cells (border cell-border cell contacts) are marked by yellow and magenta arrows, respectively. The central polar cells are indicated by red arrowheads ( K’, L’, M’, N’ ). ( O, P ) Quantification of relative E-Cad ( O ) and β-Cat ( P ) protein intensity levels in control and NiPp1 overexpressing border cell clusters shown as box-and-whiskers plots (see legend for details of plot). For E-Cad, 39 border cell-border cell contacts from eight matched control clusters and 24 border cell-border cell contacts from 16 NiPp1 clusters were measured. For β-Cat, 33 border cell-border cell contacts from seven matched control clusters and 23 border cell-border cell contacts from 15 NiPp1 clusters were measured. ***p<0.001, ****p<0.0001, unpaired two-tailed t test. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Migration, Staining, Membrane, Marker, Control, Two Tailed Test, Expressing, Generated

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A ) Close-up view of a live border cell cluster depicting how protrusions are measured. The main body of the border cell cluster is outlined (yellow circle). The protrusion length and area (green outline) were defined and measured as a cellular projection extending away from the main cluster or border cell. The schematic indicates how protrusion direction is defined. ( B, C ) Quantification of protrusion max_length ( B ) and max_area ( C ) in control versus Pp1c-RNAi border cells. Data are presented as a box-and-whiskers plot (see legend for details of plot and legend for protrusion numbers for each genotype). *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; unpaired two-tailed t test. ( D, E ) Rac-FRET in wild-type versus NiPp1 border cells (n = 22 for control, n = 18 for slbo >NiPp1). ( D ) Representative FRET images of control and NiPp1-expressing border cells, color-coded according to the heat map and FRET index. ( E ) Quantification of the total FRET index measured in control and NiPp1 border cells. Error bars represent SEM; p=0.0033 (**), unpaired t test with Welch's correction. ( F–J ) Quantification of the migration speed ( F ), number of protrusions per frame ( G ), average protrusion lifetime ( H ), average protrusion length ( I ), and average protrusion area ( J ) from videos of α-Cat- RNAi versus control border cells. For control, protrusions were measured in 14 videos (n = 51 front-directed protrusions, n = 15 side-directed protrusions, n = 2 back-directed protrusions); for α-Cat -RNAi, protrusions were measured in six videos (n = 29 front protrusions, n = 1 side protrusion, n = 9 back protrusions). Data are presented as box-and-whiskers plots (see legend for details of plot). ****p<0.0001, unpaired two-tailed t test. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Control, Two Tailed Test, Expressing, Migration

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–C ) Pp1 is required for border cell shape. ( A–B’ ) Examples of control ( A, A’ ) and NiPp1-expressing border cells ( B, B’ ). Cell shape was visualized using the membrane marker PLCδ-PH-EGFP driven by slbo- GAL4 (green). Cells were outlined ( A, B ) and measured for circularity ( C ). ( C ) Control border cells are more elongated compared to NiPp1-expressing border cells (closer to 1.0, a perfect circle). Quantification of circularity, showing all data points and the mean; 51 control border cells and 57 NiPp1-expressing border cells were measured. ****p<0.0001, unpaired two-tailed t test. ( D–G ) Pp1 restricts high levels of F-actin to the border cell cluster periphery. Egg chambers were stained for phalloidin to detect F-actin (green in D, E; white in D’, E’ ) and DAPI to visualize nuclei (white in D, E ). ( D, D’ ) Control wild-type border cells ( w 1118 ) have higher F-actin at the cluster perimeter (magenta arrows) and low levels at cell-cell contacts inside the cluster (yellow arrows). ( E, E’ ) NiPp1 overexpression increases F-actin inside the cluster at cell contacts between border cells and at cell contacts between polar cells and border cells (yellow arrows). F-actin is relatively high on the outer surfaces of border cells (magenta arrows). ( F, G ) Plot profiles of normalized F-actin (orange) and DAPI (blue) fluorescence pixel intensity (AU, arbitrary units) measured along the lines shown in ( D ) and ( E ); similar results were obtained from additional border cell clusters (n = 11 for control and n = 8 for slbo >NiPp1). ( H–I’’’’’ ) Pp1 restricts Myo-II, as visualized by Sqh-GFP, to the cluster periphery in live border cells. Stills from confocal videos of Sqh-GFP in mid-staged border cells over the course of 20 min. Enriched Sqh-GFP is marked by arrowheads. Imaging gain and other acquisition parameters were the same, except that the range of z -stacks vary slightly. Similar patterns were observed for control in n = 8 movies and n = 10 for NiPp1 overexpression. ( H–H””’ ) Control border cells . ( I–I””’ ) NiPp1 overexpression changes the dynamics of Sqh-GFP, with more Sqh-GFP located in individual border cells and at cell contacts between border cells. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Control, Expressing, Membrane, Marker, Two Tailed Test, Staining, Over Expression, Fluorescence, Imaging

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–B’’’’’ ) Stills from representative confocal videos of dynamic Sqh-GFP in early-migration borders cells over the course of 20 min. Image gain and other acquisition parameters were the same, except that the range of z -stacks may vary slightly. ( A–A’’’’’ ) Control border cells have dynamic Sqh-GFP, which is mainly restricted to the cluster perimeter. Some signal is found in the central polar cells. ( B–B’’’’’ ) NiPp1 overexpressing border cells alters the localization of Sqh-GFP, with more Sqh-GFP enriched around individual border cells. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Migration, Control

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–B’’ ) Pp1 restricts Myo-II activation to the cluster periphery. Representative images showing p-Sqh localization (white in A, B; red in A’’, ( B’’ ) and membrane GFP (PLCδ-PH-EGFP; green in A’, ( A’’, B’, B’’ ) in control ( A–A’’ ) and NiPp1 overexpressing ( B–B’’ ) border cells; DAPI labels nuclei (blue in A’’, ( B’’ ). There is an increase in p-Sqh levels (arrowheads) at the interface between border cells when NiPp1 is overexpressed. ( C ) Quantification of the mean pixel intensity of p-Sqh as a ratio of BC:NC/BC:BC. BC:NC stands for border cell-nurse cell interfaces, while BC:BC stands for border cell-border cell interfaces. N = 15 for control and n = 11 for NiPp1 overexpression. ( D–H ) Knocking down Mbs disrupts border cell migration and cluster cohesion. ( D–F ) Stage 10 control ( D ) and Mbs RNAi ( E,F ) egg chambers stained for SN to label border cells (green), phalloidin to label F-actin (red) and DAPI to label nuclei (blue). ( G ) Quantification of border cell cluster migration for matched control and Mbs-RNAi , shown as the percentage that did not complete (red), or completed (green) their migration to the oocyte (see for egg chamber schematic). ( H ) Quantification of cluster cohesion at stage 10, shown as the percentage of border cells found as a single unit (one part) or split into multiple parts (two parts, three parts,>3 parts) in control versus Mbs-RNAi border cells. ( G, H ) Each trial assayed n ≥ 61 egg chambers (total n ≥ 220 per genotype). **p<0.01; ****p<0.0001; unpaired two-tailed t test. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Activation Assay, Membrane, Control, Over Expression, Migration, Staining, Two Tailed Test

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A–B’ ) Representative processed Rho-FRET images in control ( A, A’ ) and NiPp1 overexpressing ( B, B’ ) border cells. The CFP channel ( A, B ) is shown. The FRET images ( A’, B’ ) are color-coded as indicated in the heat map. ( C ) Measurement of the total FRET index in matched control and NiPp1 overexpressing border cells. The total number of border cell clusters assayed is indicated. All genotypes are listed in .

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Control

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: Summary of the PPP family screen. Results of the targeted serine-threonine protein phosphatase RNAi screen.

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Migration, Expressing

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: ( A ) Schematic of the phenotypes and the localizations of Pp1c, F-actin, p-Sqh, and the cadherin-catenin complex during normal and Pp1-inhibited (NiPp1 expression or Pp1c-RNAi ) border cell cluster migration. ( B ) Proposed molecular pathways regulated by Pp1, which together promote cohesive collective border cell migration.

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Expressing, Migration

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet: Genotypes for figures. List of genotypes shown in the figures.

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Control

Journal: eLife

Article Title: Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration

doi: 10.7554/eLife.52979

Figure Lengend Snippet:

Article Snippet: Antibody , rabbit polyclonal anti-PPP1R8 (NiPP1) , Millipore Sigma , HPA027452; RRID: AB_1854490 , 1:100.

Techniques: Software